Origin of Escherichia coli K-12 Hfr B7. |
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| Authors: | M J Virolle J P Glugne S Bjar and J P Bouch |
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| Institution: | M J Virolle, J P Gélugne, S Béjar, and J P Bouché |
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| Abstract: | Several F' plasmids encoding resistance to tetracycline have been derived from a trg::Tn10 Hfr B7 strain of Escherichia coli K-12. One of these plasmids, JGF312, was analyzed by restriction endonuclease digestion and Southern blot hybridization to cloned chromosomal fragments. This analysis revealed that JGF312 was formed by Tn10-promoted deletion from the Tn10 insertion (31.4 min) to within the prophage rac at 30.1 min. Hfr B7 was shown to result from recombination between IS2 of F delta (33-43) and a chromosomal IS2 located within the rac-man region at 30.9 min on the genetic map. |
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