Structural relationship between tRNA2
Lys and tRNA4
Lys from mouse lymphoma cells |
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Authors: | Kirk Hayenga Charles Hedgcoth Mary Harrison Victor K Lin B J Ortwerth |
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Institution: | (1) Department of Biochemistry, Willard Hall, Kansas State University, KS 66506 Manhattan, USA;(2) Departments of Biochemistry and Ophthalmology, University of Missouri, 5201 Columbia, MO, USA |
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Abstract: | Summary Mouse lymphoma cells have three major isoaccepting lysine tRNAs. Two of these isoacceptors, tRNA2
Lys and tRNA4
Lys, were sequenced by rapid gel or chromatogram readout methods. They have the same primary sequence but differ in two modified nucleotides. tRNA4
Lys has an unmodified uridine replacing one dihydrouridine and an unidentified nucleotide, t6A*, replacing t6A. This unidentified nucleotide is not a hypomodified form of t6A. Thus, tRNA4ys is not a simple precursor of tRNA2
Lys. Both tRNAs have an unidentified nucleotide, U**, in the third position of the anticodon. Also, partial sequences of minor homologs of tRNA2
Lys and tRNA4
Lys were obtained. The distinctions between tRNA2
Lys and tRNA4
Lys may be part of significant cellular roles as illustrated by the differential effects of these isoacceptors on the synthesis by lysyl-tRNA synthetase of diadenosine-5 ,5![prime](/content/t78l88543q133t07/xxlarge8242.gif) ![prime](/content/t78l88543q133t07/xxlarge8242.gif) -P1,P4-tetraphosphate, a putative signal in DNA replication. |
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Keywords: | lysine tRNA sequence minor nucleotides precursor genes modification |
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