| 原子力显微镜对人羊膜上皮细胞的观察 |
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| 引用本文： | 陈茜,杨筱曦,蔡小芳,蔡继业.原子力显微镜对人羊膜上皮细胞的观察[J].生物技术,2010,20(2):39-42. |
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| 作者姓名： | 陈茜 杨筱曦 蔡小芳 蔡继业 |
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| 作者单位： | 1. 暨南大学化学系
2. 暨南大学附属第一医院眼科,广东广州,510632 |
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| 基金项目： | 国家自然科学基金重点项目 |
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| 摘 要： | 目的:在单细胞水平上分析人羊膜上皮细胞的超微结构及其机械性能(粘弹力、杨氏模量、硬度等),为进一步认识细胞结构与功能的关系奠定基础.方法:应用原子力显微镜(AFM)高分辨率、高灵敏度的特点,对人的羊膜上皮细胞进行观察.结果:人羊膜上皮细胞呈椭圆形,由原子力显微镜力位移曲线测量系统,可得粘弹力:1034.375±294.21 pN.硬度:1.1815±0.326mN/m,杨氏模量:16.44±4.67Kpa.结论:AFM能对人羊膜上皮细胞表面超微结构清晰地成像及提供更多更确切的表面信息及机械性能,从而增加对羊膜上皮细胞的认识.
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| 关 键 词： | 原子力显微镜(AFM) 人羊膜上皮细胞 形貌 粘弹力 硬度 扬氏模量 |
Observation of Human Amniotic Epithelial Cells by AFM |
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| CHNE Qian,YANG Xiao-xi,CAI Xiao-fang,CAI Ji-ye.Observation of Human Amniotic Epithelial Cells by AFM[J].Biotechnology,2010,20(2):39-42. |
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| Authors: | CHNE Qian YANG Xiao-xi CAI Xiao-fang CAI Ji-ye |
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| Institution: | CHNE Qian1,YANG Xiao-xi2,CAI Xiao-fang1,CAI Ji-ye1(1.Department of Chemistry,2 Department of Ophthalmology,The First Affiliated Hospital,Jinan University,Guangzhou 510632,China) |
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| Abstract: | Objective:To analyze the ultra-structure and the mechanical properties(visco-elasticity,Young's modulus,stiffness,etc.)of human amniotic epithelial cells at single-cell level.Method:Based on the high-resolution and high sensitivity of atomic force microscope(AFM),it can be used to observe the ultra-structure of human amniotic epithelial cells.Result:The studies showed that amniotic epithelial cells were oval-shaped cells.The visco-elasticity,stiffness and Young's modulus of the cell membrane can be drawn fr... |
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| Keywords: | atomic force microscope (AFM) human amniotic epithelial cells membrane ultrastructure elasticity stiffness Young's modulus |
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