Channels in the mitochondrial outer membrane: Evidence from patch clamp studies |
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Authors: | Henry Tedeschi Kathleen Walsh Kinnally |
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Institution: | (1) Department of Biological Sciences and Neurobiology Research Center, State University of New York at Albany, 12222 Albany, New York |
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Abstract: | Patch clamp techniques were applied to outer mitochondrial membranes of giant mitochondria from mice kept on a cuprizone diet or to vesicles produced by fusing membranes derived from the outer membrane ofNeurospora mitochondria. In the negative range of potentials the conductances decreased with increases in the magnitude of voltage, suggesting the closing of channels. Experiments in which mitochondria were treated with the polyanion polymethacrylate maleate styrene (1:2:3) or succinic anhydride suggest that the channels correspond to VDAC. Although sometimes conductance also decreased with increasing potential over a narrow range of positive potentials, more commonly the conductances increased. Although this phenomenon may represent a detachment of the patch, the changes in conductance are reversible, suggesting that they correspond to the formation or the opening of channels. |
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Keywords: | Mitochondrial outer membrane mitochondrial channels channels |
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