Linkage of the chicken MHC to the nucleolus organizer region visualized using non-isotopic in situ hybridization. |
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| Authors: | M Dominguez-Steglich C Auffray M Schmid |
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| Affiliation: | Department of Human Genetics, University of Würzburg, Germany. |
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| Abstract: | Linkage of the chicken major histocompatibility complex (B-complex) to the nucleolus organizer regions (NOR) has been confirmed in situ. Nonradioactive hybridization to metaphase chromosomes using a pool of biotinylated cDNA probes for the B-F, B-L beta, and B-G genes of the B-complex gave positive signals on one or a pair of microchromosomes. Subsequent staining with silver nitrate proved these microchromosomes to be the NOR-bearing pair. The value of employing nonisotopic in situ hybridization techniques for mapping of genes and gene complexes, especially when dealing with microchromosomes, is stressed. |
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