Heavy metal-nucleotide interactions. 15. Reactions of calf thymus DNA with the electrophiles methylmercury(II) nitrate, cis-dichlorodiammineplatinum(II), and trans-dichlorodiammineplatinum(II) studied using Raman difference spectroscopy. Evidence for the formation of c-DNA upon metalation |
| |
| Authors: | O'Connor T Bina M McMillin D R Moller Haley M R Tobias R S |
| |
| Affiliation: | Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. |
| |
| Abstract: | This study details the reactions of the electrophiles CH3Hg(NO3), cis-[PtCl2(NH3)2] (cis-DDP) and trans-[PtCl2(NH3)2] (trans-DDP) with calf thymus DNA using Raman and Raman difference spectroscopy. The order of CH3Hg(II) binding to calf thymus DNA is G > T > C > A. The electrophilic attack of cis- and trans-DDP on calf thymus DNA produces different orders of binding: cis-DDP-G>C approximately AT, trans-DDP-G approximately C approximately AT. The reaction of CH3Hg(II) with DNA results in a decrease in the percentage of B-form DNA. whereas the reactions of cis- and trans-DDP with DNA decrease the percentage of B-DNA and cause the formation of C-DNA structure. |
| |
| Keywords: | DNA structure: Antitumor drug Mutagen Roman spectroscopy Methylmercury(II) Dichlorodiammineplatinum(II) |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
