Detection of quantitative trait loci for carcass traits in the pig by using AFLP |
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Authors: | Klaus Wimmers Eduard Murani Siriluck Ponsuksili Martine Yerle Karl Schellander |
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Institution: | (1) Institute of Animal Breeding Science, University of Bonn, 53115 Bonn, Germany, DE;(2) Laboratoire de Genetique Cellulaire, INRA, 31326 Castanet Tolosan, France, FR |
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Abstract: | For evaluation of the suitability of Amplified Fragment Length Polymorphism (AFLP) for detection of quantitative trait loci
in farm animals, a combination of AFLP and selective genotyping has been applied as a rapid screening method for marker–QTL
associations. Focusing on loci affecting eye muscle area, six extreme discordant sib pairs were selected from a Duroc × Berlin
Miniature Pig F2 experimental cross and examined by using 48 AFLP primer combinations. Two prominent AFLP markers were converted into simple
codominant PCR markers (STS-Bo1 and STS-Bo3) and assigned to Sscr4 by physical and linkage mapping. Single marker analysis indicated association of the STS markers with
a putative QTL influencing eye muscle area. Interval mapping confirmed the presence of a significant QTL for eye muscle area
(Pgenomewide < 0.01) on the Sscr4, with STS-Bo1 being the closer marker. At the same location, significant effects (Pgenomewide < 0.01) on carcass length and backfat thickness were also detected. Our results demonstrate the capability of the combination
of AFLP analysis and selective genotyping as a method for detection of genome regions containing QTL in livestock. |
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