社鼠（Niviventer confucianus）线粒体基因组全序列分析

社鼠（Niviventer confucianus）属于啮齿目（Rodentia）、鼠科（Muridae）、白腹鼠属（Niviventer）,关于该物种的分子系统学研究极少。为获取社鼠线粒体基因组全序列,提取其基因组总DNA,参照近缘物种线粒体基因组全序列设计34对特异性引物,利用PCR扩增全部片段后进行测序,之后对其基因组组成及结构特点进行了初步分析。结果表明,社鼠线粒体基因组全序列长16 281 bp（GenBank收录号：KJ152220）,包含22个tRNA基因、13个蛋白质编码基因、2个rRNA基因和1个非编码控制区;基因组核苷酸组成为34.0%A、28.6%T、24.9%C、12.5%G。将所得序列与社鼠近缘物种（川西白腹鼠、小家鼠、褐家鼠）的线粒体全基因组进行比较,结果显示,四个物种的线粒体基因组虽然在基因组大小、部分tRNA二级结构、部分蛋白质编码基因的起始或终止密码子及控制区长度和碱基组成上有差异,但基因组结构和序列特征方面都具有较高的相似性。四个物种线粒体全基因组间的遗传距离显示,社鼠与川西白腹鼠距离最近,而与小家鼠距离最远。该研究为利用线粒体全基因组信息进行啮齿类分子系统学研究提供了有价值的资料。

Sequence Analysis of the Complete Mitochondrial Genome of Niviventer confucianus

Niviventer confucianus belongs to genus Niviventer, family Muridae, order Rodentia, and it is involved in few molecular phylogenetic studies. The complete mitochondrial genome （mitogenome） sequence of N. confucianus was firstly obtained with PCR and sequencing techniques using 34 pairs of specific primes. The mitogenome （the GenBank accession number： KJ152220） was 16 281 bp in length and the nucleotide composition was 34.0% A, 28.6% T, 24.9% C and 12.5% G. The mitogenome consisted of 13 protein-coding genes, 22 tRNAs, 2 rRNAs and 1 control region, and the genome organization and sequence characters didn＇t differ greatly from those ofmitogenomes of N. excelsior, Mus musculus, and Rattus norvegicus, though small difference existed. The genetic distance among mitogenomes of four species showed that the relationship between N. confucianus and N. excelsior was closest, while the relationship between N. confucianus and M. musculus was farthest. This work provides valu- able data for phylogenetic analysis of rodents with complete mitogenome information.