Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility |
| |
Authors: | Muhammad Harunur Rashid Akio Kuroda Junichi Sekiguchi |
| |
Institution: | Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan |
| |
Abstract: | Abstract The purified autolytic endo- β - N -acetylglucosaminidase of Bacillus subtilis AC327 was cleaved with cyanogen bromide, and the N-terminal amino acid sequence of one of the peptide fragments was determined. Then, a DNA fragment containing a part of the glucosaminidase gene was cloned into Escherichia coli JM109 using synthetic oligonucleotides as probes whose sequences had been deduced from the N-terminal amino acid sequence. Zymographic analysis showed that the resultant glucosaminidase-deficient strain lacked a 35-kDa lytic band in addition to a 90-kDa lytic one corresponding to the glucosaminidase. A double mutant strain deficient in the major two autolysins (amidase and glucosaminidase) exhibited greatly impaired motility on a swarm plate whereas the single mutant strains were motile. |
| |
Keywords: | Bacillus subtilis Autolysin Glucosaminidase Amidase Motility |
|