Regeneration of somatic embryos from sweet orange (C. sinensis) protoplasts using semi-permeable membranes |
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Authors: | Randall P Niedz |
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Institution: | (1) Agricultural Research Service, US Horticultural Research Laboratory, 2001 South Rock Road, Ft. Pierce, FL 34945-3030, USA |
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Abstract: | Sweet orange (C. sinensis L. Osbeck) protoplasts were isolated from nucellar-derived embryogenic callus, cultured in alginate beads for 5–30 days,
and the resulting p-calli released by liquefaction and cultured on semi-permeable membranes overlaid on MT culture medium.
Somatic embryos did not develop from 5- to 10-day-old p-calli but did develop from 15-, 20-, 25-, and 30-day-old p-calli.
There were no significant differences in the numbers of embryos produced among the 15- to 30-day-old p-calli and no abnormal
embryo morphologies were observed. The minimum size of p-calli to form embryos was 77.84 μm in diameter. Embryos were smaller
from p-calli than those produced from embryogenic callus; p-calli-derived embryos ranged in size between 0.5 and 0.8 mm, while
embryos derived from embryogenic callus ranged between 1 and 2 mm. |
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Keywords: | cellulose acetate citrus protoplast culture somatic embryogenesis |
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