Evaluation of growth hormone production from GH1 cells in vitro: Effect of culture media and time in culture |
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Authors: | W E Bolton A E Boyd III |
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Institution: | (1) Baylor College of Medicine, 1200 Moursund, 77030 Houston, Texas |
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Abstract: | Summary Growth hormone production by a rat pituitary tumor cell line (GH1) was measured during lag, exponential, and plateau phases
of growth in different culture media. Growth hormone secretion was low during lag and early exponential phase; it increased
late in the exponential phase and continued to increase during the plateau phase. This biphasic pattern of growth hormone
production was observed in all media and sera utilized. Both the doubling time and growth hormone production were influenced
by the choice of media and sera. In addition, the length of time in culture affected the growth fraction with passage level
40 GH1 cells having a 79% growth fraction, whereas the growth fraction of passage level 100 cells was 95%. Using the population
doubling time as a criterion for a choice of medium, F-10 medium supplemented with 20% fetal bovine serum consistently yielded
the most rapid doubling time (32 hr), whereas Dulbecco's MEM supplemented with 15% horse serum and 2.5% fetal bovine serum
yielded the greatest plateau cell density.
Growth hormone secretion and the population doubling times were directly related to culture conditions including length of
time in culture, choice of tissue culture media, choice of sera, and the phase of cell growth (lag, exponential or plateau). |
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Keywords: | GH1 cell culture growth hormone nutrition |
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