Identification and mapping of net form of net blotch resistance in South African barley |
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| Authors: | Anke Martin Gregory J. Platz Daniel de Klerk Ryan A. Fowler Francois Smit Francois G. Potgieter Renée Prins |
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| Affiliation: | 1.Centre for Crop Health,University of Southern Queensland,Toowoomba,Australia;2.Queensland Department of Agriculture and Fisheries, Hermitage Research Facility,Warwick,Australia;3.South African Barley Breeding Institute,Caledon,South Africa;4.CenGen (Pty) Ltd,Worcester,South Africa;5.Department of Plant Sciences,University of the Free State,Bloemfontein,South Africa |
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| Abstract: | Net form of net blotch (NFNB) caused by the fungus Pyrenophora teres f. teres is an economically important foliar disease of barley (Hordeum vulgare) in southern and eastern Africa. Little attention has been given to disease resistance breeding, and knowledge about the presence of NFNB resistance in breeding lines is limited. Deploying resistance into varieties used in this region is important for future control of the disease. We have identified NFNB disease resistance in existing South African breeders’ lines and have mapped the resistance in line UVC8. Six different trials, three conducted in South Africa and another three in Australia, were used to identify resistance QTL. A major QTL was identified on chromosome 6H having a LOD score of 40.5 and 55% of the phenotypic variance explained. Kompetitive Allele Specific PCR (KASP?) markers were designed for this QTL region. These and microsatellite markers can now be used to routinely select for NFNB resistance. |
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