Gibberellin-induced Yeast Sporulation in Relation to RNA and Protein Metabolism |
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| Authors: | Seiichiro Kamisaka Yoshio Masuda Naohiko Yanagishima |
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| Institution: | Laboratory of Cell Biology, Faculty of Science Osaka City University, Sumiyoshi-ku, Osaka, Japan |
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| Abstract: | Gibberellic acid (GA) promoted sporulation in yeast when added to the sporulation medium. When added together with GA, metabolic inhibitors of RNA synthesis such as 8-azaguanine, 2-thiouracil, and actinomycin D suppressed selectively the promoting effect of GA on sporulation. The effect of 8-azaguanine and 2-thiouracil was alleviated by simultaneous addition of guanine and uracil, respectively. The promoting effect of GA on sporulation was also suppressed by inhibitors of protein synthesis such as ethionine, chloramphenicol, and puromycin. Methionine eliminated the inhibitory effect of ethionine on the GA action. |
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