| 一种适于转基因水稻PCR检测的微量DNA快速提取法 |
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| 引用本文： | 许明,程祖锌,黄志伟,杨志坚,郑金贵.一种适于转基因水稻PCR检测的微量DNA快速提取法[J].生物技术通报,2010(3). |
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| 作者姓名： | 许明 程祖锌 黄志伟 杨志坚 郑金贵 |
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| 作者单位： | 农业部海峡两岸农业技术合作中心,福建农林大学农产品品质研究所,福州,350002 |
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| 基金项目： | 国家自然科学基金项目，转基因生物新品种培育重大专项，福建省科技厅重大专项 |
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| 摘 要： | 对已报道的小麦基因组DNA快速提取方法的部分步骤进行了简化,在水稻上进行了尝试。结果表明,简化法提取的水稻基因组DNA完整性好,PCR扩增效果与试剂盒提取法无明显的差异,结果稳定可靠;而且整个提取过程操作简单、花费时间少,样品用量少,仅需5-10mg,适用于大规模转基因水稻的PCR检测。
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| 关 键 词： | 转基因水稻 PCR检测 基因组DNA 快速提取 |
A Rapid Trace DNA Extraction Method Suitable for the PCR Detection of Transgenic Rice |
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| Xu Ming,Chen Zuxin,Huang Zhiwei,Yang Zhijian,Zheng Jingui.A Rapid Trace DNA Extraction Method Suitable for the PCR Detection of Transgenic Rice[J].Biotechnology Bulletin,2010(3). |
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| Authors: | Xu Ming Chen Zuxin Huang Zhiwei Yang Zhijian Zheng Jingui |
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| Institution: | Xu Ming Chen Zuxin Huang Zhiwei Yang Zhijian Zheng Jingui(Cross-Strait Agricultural Technology Cooperation Center,the Ministry of Agriculture,Agricultural Product Quality Institute,Fujian Agriculture , Forestry University,Fuzhou 350002) |
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| Abstract: | The reported method of wheat rapid DNA extraction was simplified at some steps and then applied to rice in this study.The results showed that rice genomic DNA extracted by this simplified method was integral and the PCR amplification using it as template was stable and reliable,showing no significant difference from plant genomic DNA extraction kit method.Furthermore,the whole extraction procedure is simple,takes less time,and requires a small amount of plant sample about 5-10 mg.Therefore this method is su... |
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| Keywords: | Transgenic rice PCR detection Genomic DNA Rapid extraction |
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