Oxidative stress-induced apoptosis prevented by trolox |
| |
Authors: | Virginia J. Forrest Yuan-Hsu Kang David E. McClain Douglas H. Robinson Narayani Ramakrishnan |
| |
Affiliation: | a Naval Medical Research Institute, Bethesda, MD, USA b Armed Forces Radiobiology Research Institute, Bethesda, MD, USA |
| |
Abstract: | The ability of oxidative stress to induce apoptosis (programmed cell death), and the effect of Trolox, a water soluble vitamin E analog, on this induction were studied in vitro in mouse thymocytes. Cells were exposed to oxidative stress by treating them with 0.5–10 μM hydrogen peroxide (H2O2) for 10 min, in phosphate-buffered saline supplemented with 0.1 mM ferrous sulfate. Cells were resuspended in RPMI 1640 medium with 10% serum and incubated at 37°C under 5% CO2 in air. Electron microscopic studies revealed morphological changes characteritic of apoptosis in H2O2-treated fragmented the DNA in a manner typical of apoptotic cells, producing a ladder pattern of 200 base pair increments upon agarose gel electrophoresis. The percentage of DNA fragmentation (determined fluorometrically) increased with increasing doses of H2O2 and postexposure incubation times. Pre- or posttreatment of cells with Trolox reduced H2O2-induced DNA fragmentation to control levels and below. The results indicate that oxidative stress induces apoptosis in thymocytes, and this induction can be prevented by Trolox, a powerful inhibitor of membrane damage. |
| |
Keywords: | Apoptosis Oxidative stress DNA fragmentation Trolox Vitamin E Hydrogen peroxide Thymocytes Membrane damage Oxygen free radicals |
本文献已被 ScienceDirect 等数据库收录! |
|