Rhamnogalacturonan acetylesterase: a novel enzyme from Aspergillus aculeatus,specific for the deacetylation of hairy (ramified) regions of pectins |
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Authors: | M J F Searle-van Leeuwen L A M van den Broek H A Schols G Beldman A G J Voragen |
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Institution: | (1) Department of Food Science, Wageningen Agricultural University, Biotechnion, Bomenwag 2, NL-6703 HD Wageningen, The Netherlands |
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Abstract: | Rhamnogalacturonan acetylesterase, able to specifically hydrolyse the acetyl asters present in modified hairy (ramified) regions (MHR) of apple pectin, was identified. The enzyme removed about 70% of the total acetyl groups in MHR. This acetylesterase did not cause the release of acetyl groups from a range of other acetylated substrates, either synthetic or extracted from plants, including the acetylated smooth regions present in beet pectin. Pretreatment of pectic polysaccharides in order to remove arabinose side chains had no effect on the acetyl release, wor was an effect found on the rate or degree of acetyl release, when the purified acetylesterase was combined with pectolytic enzymes, pectin methylesterase or arabinanases.
Correspondence to: A. G. J. Voragen |
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