A multicopy suppressor screening approach as a means to identify antibiotic resistance determinant candidates in <Emphasis Type="Italic">Yersinia pestis</Emphasis> |
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Authors: | Karen L Stirrett Julian A Ferreras Sebastian M Rossi Richard L Moy Fabio V Fonseca Luis EN Quadri |
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Institution: | (1) Department of Microbiology and Immunology, Weill Medical College of Cornell University, 1300 York Avenue, 10021 New York, USA;(2) Facultad de Ciencias Exactas, Quimicas y Naturales, Universidad Nacional de Misiones, Felix de Azara 1552, N3300LQH Posadas, C.P., Argentina;(3) Medical College of Georgia, Vascular Biology Center, 1459 Laney Walker Boulevard, 3201B Augusta,Georgia 30912, CB, USA |
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Abstract: | Background
Yersinia pestis is the causative agent of plague and a potential agent of bioterrorism and biowarfare. The plague biothreat and the emergence
of multidrug-resistant plague underscore the need to increase our understanding of the intrinsic potential of Y. pestis for developing antimicrobial resistance and to anticipate the mechanisms of resistance that may emerge in Y. pestis. Identification of Y. pestis genes that, when overexpressed, are capable of reducing antibiotic susceptibility is a useful strategy to expose genes that
this pathogen may rely upon to evolve antibiotic resistance via a vertical modality. In this study, we explored the use of
a multicopy suppressor, Escherichia coli host-based screening approach as a means to expose antibiotic resistance determinant candidates in Y. pestis. |
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