Inhibition of Alzheimer amyloid aggregation with sulfated glycopolymers |
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Authors: | Miura Yoshiko Yasuda Kikuko Yamamoto Kiyofumi Koike Mihoko Nishida Yoshihiro Kobayashi Kazukiyo |
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Institution: | Department of Molecular Design and Engineering, Graduate School of Engineering, Nagoya University, Nagoya, Japan. miuray@jaist.ac.jp |
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Abstract: | Glycopolymers carrying sulfated saccharides with modest sugar contents (11% and 28%) were found to suppress the formation of amyloid fibrils by amyloid beta peptides (Abeta(1-42), Abeta(1-40), and Abeta(25-35)), as evaluated by thioflavin T assays and atomic force microscopy observation. Circular dichroism spectra showed that the conformation of amyloid beta peptides depended on the glycopolymer additives, and that the glycopolymer additives reduced the beta-sheet contents. Neutralization activity was confirmed by in vitro assay with HeLa cells. The sulfate group and the appropriate sugar contents were essential for the inhibitory effect. |
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