Polyelectrolyte-coated microcapsules and their potential applications to biotechnology |
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Authors: | Etsuo Kokufuta |
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Institution: | (1) Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan |
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Abstract: | Polyelectrolyte-coated microcapsules can be prepared by adsorption of polyions onto microcapsule surfaces in aqueous solutions
under appropriate pH and ionic conditions. The resulting polyelectrolyte-coated microcapsules provide a promising tool for
studying pH-induced configurational changes in polyions adsorbed onto hydrophobic membranes (capsule walls). An interesting
application of polyelectrolyte-coated microcapsules is the pH-sensitive on/off control of microencapsulated enzyme reactions
through alterations in the substrate permeability of the capsule wall by pH-conditioned configurational changes in the adsorbed
polyion layer. This paper presents an overview of pH-induced conformational changes of polyelectrolytes in solutions, preparation
of polyelectrolyte-coated microcapsules with an immobilized enzyme, and on/off control of the respective enzyme reactions
by pH adjustment.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | conformational changes enzyme reactions microencapsulated enzymes polymer-coated microcapsules polyelectrolytes substrate permeability on/off control |
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