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Hazards in the use of Cibacron Blue F3GA in studies of proteins. |
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| Authors: | B H Weber K Willeford J G Moe D Piszkiewicz |
| Institution: | 1. Department of Chemistry, California State University, Fullerton, California 92634 USA;2. Institute of Molecular Biology, California State University, Fullerton, California 92634 USA;3. Department of Biological Chemistry, University of California, Irvine, California 92717 USA;4. Department of Chemistry, Duquesne University, Pittsburgh, Pennsylvania 15219 USA |
| Abstract: | Cibacron Blue F3GA from several commercial sources is shown to be heterogeneous. This crude dye inactivates both phosphoglycerate kinase and isoleucyl-tRNA synthetase. Purification of Cibacron Blue F3GA to homogeneity results in a dramatic decrease in inactivation of these enzymes. The inactivation is shown to be due to covalent modification of phosphoglycerate kinase and probably isoleucyl-tRNA synthetase by a minor component present in crude Cibacron Blue F3GA. |
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