Development of 11 EST-SSRs for Japanese white birch, Betula platyphylla var. japonica and their transferability to related species |
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| Authors: | Yoshiaki Tsuda Saneyoshi Ueno Janita Ranta Katja Salminen Yuji Ide Kenji Shinohara Yoshihiko Tsumura |
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| Institution: | (1) Tree Genetics Laboratory, Department of Forest Genetics, Forestry and Forest Products Research Institute (FFPRI), 1 Matsunosato, Tsukuba Ibaraki, 305-8687, Japan;(2) Department of Biology, University of Oulu, P.O. Box 3000, 90014, Oulu, Finland;(3) Laboratory of Forest Ecosystem Studies, Department of Ecosystem Studies, Graduate School of Agriculture and Life sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo, Tokyo 113-8657, Japan;(4) Department of Molecular and Cell Biology, Forestry and Forest Products Research Institute (FFPRI), 1 Matsunosato, Tsukuba Ibaraki, 305-8687, Japan |
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| Abstract: | Simple sequence repeat (SSR) markers were developed for Japanese white birch, Betula platyphylla var. japonica, using previously designed primer pairs derived from expressed sequence tags (ESTs). Out of 98 unpublished primer pairs,
35 yielded clear PCR amplification products, 11 of which revealed polymorphism in eight individuals sampled across the species’
range. The number of alleles detected and the expected heterozygosity ranged from 1 to 10 and 0.000 to 0.857, respectively,
when these 11 loci were examined in 24 individuals from a single B. platyphylla var. japonica population. In cross-species transferability tests most of the 11 loci were also polymorphic in three other Betula species examined, but not B. maximowicziana. We have now developed a total of 25 polymorphic EST-SSRs for the genus Betula (including 14 we previously developed), which are likely to be highly useful in studies of various aspects of population
genetics, including hybridization dynamics, in the genus. |
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