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Activation of polycyclic hydrocarbons in Reuber H4-II-E hepatoma cells an in vitro system for the induction of SCEs |
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| Authors: | R. G. Dean G. Bynum D. Jacobson-Kram E. Hadley |
| Affiliation: | 1 Section on Cellular Aging and Genetics, Laboratory of Cellular and Molecular Biology, Gerontology Research Center, National Institute on Aging, Baltimore, MD, USA 3 George Washington University School of Medicine, Department of Radiology, Washington, DC, USA 4 National Institute on Aging, NIH, Bethesda, MD, U.S.A. |
| Abstract: | Activation of polycyclic aromatic hydrocarbons (PAHs) was examined in the Reuber H4-II-E established cell line without the use of exogenous enzyme preparations. Metabolism of PAHs to genotoxic products was determined by the induction of sister-chromatid exchanges (SCEs). The induction of SCEs followed a dose-response pattern with plateaus at high doses of PAH. The effects of metabolic enzyme inducers (3-methylcholanthrene, phenobarbital, Aroclor 1254) and the epoxide hydrase inhibitor 1,1,1-trichloropropylene oxide were assessed as changes in SCE induction and enhanced production of water-soluble metabolites. Results indicate that Reuber H4-II-E cells can be employed in the testing of carcinogens activated by the P1-450 monooxygenase system and would be a useful in vitro system for the study of mechanisms of metabolic induction and their effect on genetic toxicity. |
| Keywords: | BP DMBA MC 3-methylcholanthrene PAHs polycyclic aromatic hydrocarbons PB phenolbarbitol TCPO 1,1,1-trichloropropylene oxide |
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