Modification of Lipase by Polyethylene Glycol |
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| Authors: | M Basri A B Salleh K Ampon W M Z Yunus C N A Razak |
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| Institution: |
a Enzyme and Microbial Technology Research, Fakulti Sains & Pengajian Alam Sekitar, Universiti Pertanian Malaysia, Serdang, Malaysia |
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| Abstract: | Lipase was modified using polyethylene glycol activated by p-nitrochloroformate. The hydrolytic activity of the polyethylene glycol-derivatised lipase (PEG-lipase) was relatively low compared with that of the unmodified enzyme in aqueous system. The esterification activity, however, was enhanced following the modification. The rate of esterification of butyric acid was higher than that of oleic acid. Benzene was the best solvent for the esterification reaction. |
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| Keywords: | Lipase modified polyethylene glycol |
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