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Herbicide tolerant regenerates of potato
Authors:G. Wersuhn  K. Kirsch  R. Gienapp
Affiliation:(1) Sektion Biologie, Humboldt-Universität Berlin, Invalidenstrasse 43, DDR-1040 Berlin, German Democratic Republic
Abstract:Summary Culture-derived plants and cell cultures of potato (Solanum tuberosum L.) respond to the application of the herbicides SYS 67 ME (MCPA) and OMNIDEL (Na-2,2-dichloropropionate) in a comparable fashion. By gradually increasing the herbicide concentration, cell lines were developed which tolerated 50 mg/l of ME or 300 mg/l of OMNIDEL. Any further increase in concentration resulted in the death of all cell cultures. From cell cultures that had been able to grow on media supplemented with 30 mg/l of ME, regenerate plants were obtained that were also tolerant to this concentration. This new trait was retained even after repeated vegetative propagation of the plants.
Keywords:In-vitro selection  Herbicide tolerance  Regeneration  Tolerance test  Solanum tuberosum L.
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