The synthesis of proteolipid protein by isolated rat liver mitochondria |
| |
Authors: | J P Burke D S Beattie |
| |
Affiliation: | Department of Biochemistry, Mount Sinai School of Medicine of the City University of New York, New York, N.Y. 10029 USA |
| |
Abstract: | About 15% of the total (3H)leucine incorporated into protein by isolated rat liver mitochondria could be extracted by chloroform:methanol. This incorporation was inhibited by chloramphenicol and carbomycin, both specific inhibitors of mitochondrial protein synthesis. SDS-gel electrophoresis of the mitochondrial membrane revealed 6–7 labeled bands. Label in the proteolipid fraction was present mainly in a band of 40,000 molecular weight. Several labeled bands observed in gels of the mitochondrial membrane were not removed or changed by extraction with chloroform:methanol suggesting that some, but not all, of the proteins synthesized by rat liver mitochondria are proteolipids. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|