Histochemical demonstration of G6PDH- and SDH-activity on cultured fibroblastoid cells from rat pancreas using a "membrane technique" |
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Authors: | G Weiss G Vergani S Pentz |
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Abstract: | The applied "membrane technique" represents a simple and time-saving method to provide evidence for presence of enzyme activities in single cultured cells. Substrates for G6PDH and SDH are filled on a semipermeable membrane stretched over a cylinder of plastic. The cells are cultured on a polyester foil and incubated for the enzyme staining reaction on the membrane. Enzyme products can be visualized under the microscope within the cells in definite areas. |
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