Saccharide production from methanol by transposon 5 mutants derived from the extracellular polysaccharide-producing bacterium Methylobacillus sp. strain 12S |
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| Authors: | T Yoshida M Horinouchi H Habe Y Ayabe T Yamaguchi N Shibuya H Nojiri H Yamane T Omori |
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| Institution: | (1) Biotechnology Research Center, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan e-mail: aseigyo@mail.ecc.u-tokyo.ac.jp Tel.: +81-3-58413067 Fax: +81-3-58418030, JP;(2) Laboratory of Microbiology, Institute of Physical and Chemical Research (Riken), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan, JP;(3) Department of Biotechnology, National Institute of Agrobiological Resources, 2-1-2 Kannondai, Tukuba, Ibaraki 305-8602, Japan, JP |
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| Abstract: | A CH3OH-utilizing bacterium that has the ability to produce extracellular polysaccharide (EPS) was isolated from a soil sample,
and was identified as the obligate methylotroph Methylobacillus sp. strain 12S on the basis of its 16S rDNA sequence and growth-substrate specificity. The EPS produced by strain 12S was
purified and the sugar composition was analysed by GC-MS and HPLC to reveal that the EPS was a heteropolymer composed of glucosyl,
galactosyl, and mannosyl residues in the molar ratio 3:1:1. In order to produce mono- and/or oligosaccharides by single-step
fermentation from CH3OH, stain 12S was mutagenized by transposon 5. Among eleven EPS-deficient mutants, three strains were found to accumulate significant amounts of reducing sugars in the
media. The amounts of the reducing sugars produced by the mutants (>ca. 700 mg glucose equivalent/l) were >11–22 times higher
than those produced by the wild-type strain (<ca. 60 mg glucose equivalent/l). The GC-MS analysis showed that all the mutants
accumulated glucose, erythrose, threose and a disaccharide-like compound in the media.
Received: 25 August 1999 / Received revision: 15 March 2000 / Accepted: 24 March 2000 |
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