Leukotriene B4 production by stimulated whole blood: comparative studies with isolated polymorphonuclear cells |
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| Authors: | P Gresele J Arnout M C Coene H Deckmyn J Vermylen |
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| Institution: | 1. Center for Thrombosis and Vascular Research, Univ. of Leuven, B-3000 Leuven, Belgium;2. Dept. of Life Sciences, Janssen Pharmaceutica, B-2340 Beerse, Belgium;1. Cutaneous Biology Research Center, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA;2. Genomics Institute of the Novartis Research Foundation, San Diego, CA 92121, USA;3. Massachusetts General Hospital Cancer Center, Harvard Medical School, Boston, MA 02114, USA;4. Division of Signal Transduction, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA;1. Department of Applied Pharmacology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, 890-8544, Japan;2. Department of Pharmacy, School of Pharmacy, Hyogo Medical University, 1-3-6 Minato-jima, Kobe, Hyogo, 650-8530, Japan;3. Department of Oral Anatomy and Cell Biology, Graduate School of Medical and Dental Sciences, Kagoshima University, 8-35-1 Sakuragaoka, Kagoshima, 890-8544, Japan;4. Division of Radiation Biology and Medicine, Faculty of Medicine, Tohoku Medical and Pharmaceutical University, 1-15-1 Fukumuro, Miyagino-ku, Sendai, Miyagi, 983-8536, Japan;5. Department of Pharmacology, School of Medicine, Hyogo Medical University, 1-1 Mukogawa-cho, Nishinomiya, Hyogo, 663-8501, Japan |
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| Abstract: | A new method was developed to study leukotriene B4 (LTB4) production by stimulated whole blood. The calcium ionophore A23187 and serum-treated zymosan induced LTB4 production, measured by radioimmunoassay, in a dose- and time-dependent manner. The pattern of LTB4 production by whole blood differed markedly from that observed with isolated, purified polymorphonuclear leukocytes. Higher levels of LTB4 were reached and maintained in whole blood. The system allowed to detect drug effects on LTB4 synthesis in vitro. This new method to study the synthesis of LTB4 takes into account the complex interactions between different cell types which can modulate LTB4 metabolism. |
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