Variability in gold bead density in cells |
| |
Authors: | I Hammel M Elmalek M Castel M Kalina |
| |
Institution: | (1) Department of Pathology, Sackler School o Medicine, Tel-Aviv University, 69978 Tel-Aviv, Israel;(2) Department of Histology and Cell Biology, Sackler School o Medicine, Tel-Aviv University, 69978 Tel-Aviv, Israel;(3) Department of experimental Zoology, The Hebrew University, Jerusalem, Israel |
| |
Abstract: | Summary Variability in gold bead distribution between individual cells was demonstrated in both pituitary melanotrophic cells immunocytochemically reacted for ACTH and in neurohypophysia terminals reacted for oxytocin-neurophysin. Gold beads were confined to the secretory granules compartment of both tissues. Density of gold beads in melanotrophic cells reacted for ACTH varied from 100–480 gold beads/m2. A much narrower range of gold beads distribution (460–900 gold beads/m2) was observed in axons of the neurohypophysis reacted with anti-oxytocin-neurophysin. These results indicate that the labeling density varies from cell to cell (as well as axon terminals) within morphologically homogeneous population. Thus, it may reflect certain physiological differences between cells. A suggestion is being made that mean gold bead density coefficient of variation should be calculated by comparison between individual cells. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|