Molecular Cloning and Characterization of Transferrin from Wax Moth, Galleria mellonella |
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Authors: | Jikhyon HAN Kyung-Pil NAM Sook Jae SEO Chi-Young YUN |
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Affiliation: | Department of Biology, Daejeon University, Daejeon 300-716, Korea;Division of Life Science, Gyeongsang National University, Jinju 660-701, Korea |
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Abstract: | Complete mRNA sequence of transferrin from Galleria mellonella was obtained, and compared with those of other species. Until now, two types of insect transferrin were reported. Transferrins in cockroach and termite have two iron binding sites while those in most other insect groups, studied for the protein, have only one. It was suggested that the presence of two types of transferrin was related with transferrin evolution, because vertebrate transferrins have two iron binding sites, called N and C terminal lobe. It was shown that G. mellonella transferrin also has only one iron binding site (N terminal lobe), and the deduced amino acid sequence was most similar to those of Manduca sexta and Bombyx mori. |
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Keywords: | Transferrin Wax moth Galleria mellonella cDNA cloning |
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