1,2-Diacylglycerols do not potentiate the action of phospholipases A2 and C in human platelets |
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Authors: | S P Watson B R Ganong R M Bell E G Lapetina |
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Institution: | 1. Department of Molecular Biology, The Wellcome Research Laboratories, 3030 Cornwallis Road, Research Triangle Park, North Carolina 27709 USA;2. Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710 USA |
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Abstract: | 1,2-Diacylglycerol has recently been reported to potentiate the ability of phospholipases A and C to hydrolyze phospholipids in a cell-free system. The present study has been undertaken to investigate whether 1,2-diacylglycerol can also perform this function in intact cells using the platelet as a test system. Exogenous 1-oleoyl-2-acetyl-glycerol ( OAG ) and 1,2- didecanoylglycerol , at concentrations sufficient to produce maximal phosphorylation of a 40,000 dalton protein, caused no significant formation of 3H]inositol phosphates and 32P]phosphatidic acid (products of phospholipase C activation) or 14C]arachidonic acid metabolites and lysophosphatidyl3H]inositol (products of phospholipase A2 activation). These data therefore imply that 1,2-diacylglycerols do not potentiate the actions of phospholipases A2 and C in intact platelets at concentrations that are physiologically relevant. |
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Keywords: | OAG 1-oleoyl-2-acetyl-glycerol |
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