Authentic and recombinant bilirubin oxidases are in different resting forms |
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| Authors: | Sakurai Takeshi Zhan Lei Fujita Takahiro Kataoka Kunishige Shimizu Atsushi Samejima Tatsuya Yamaguchi Shotaro |
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| Institution: | Department of Chemistry, Faculty of Science, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan. ts0513@kenroku.kanazawa-u.ac.jp |
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| Abstract: | Myrothecium verrucaria bilirubin oxidase expressed in Aspergillus oryzae is in a resting form different from that of the authentic bilirubin oxidase, but reaches the resting form of the authentic enzyme after one cycle of reduction and reoxidation with dioxygen as shown by the absorption and electron paramagnetic resonance spectra. |
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