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Backbone NMR assignments of DFP-inhibited mature subtilisin E
Authors:Yu-Jen Chen  Kuen-Phon Wu  Seho Kim  Liliana Falzon  Masayori Inouye  Jean Baum
Institution:(1) Department of Biochemistry, Robert-Wood-Johnson Medical School, 675 Hoes Ln, Piscataway, NJ 08854, USA;(2) Department of Chemistry and Chemical Biology, Rutgers University, 610 Taylor Rd, Piscataway, NJ 08854, USA;(3) BioMaPS Institute for Quantitative Biology, Rutgers University, 610 Taylor Rd, Piscataway, NJ 08854, USA
Abstract:Here we report the backbone chemical shifts of the DFP-inhibited mature subtilisin E, which was uniformly labeled by 13C, 15N with a supplement of excess calcium.
Keywords:Subtilisin  Intramolecular chaperone  Serine protease  Propeptide
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