High-performance liquid chromatography and its use in cytokinin determination in Agrobacterium tumefaciens B6

Seven natively occurring cytokinins were separated using a high-performanceliquid chromatography system. The analysis was completed in23 min. The applicability of the system is shown in determiningthe cytokinin contents in the plant pathogen Agrobacterium tumefaciensB6, which was found to contain 66 ng of N⁶-(²-isopentenyl) adenineper g bacteria. High-performance liquid chromatography and gasliquid chromatography are compared and evaluated for cytokinindetermination. (Received May 7, 1976; )