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The selective visualization of lignin peroxidase,manganese peroxidase and laccase,produced by white rot fungi on solid media
Authors:Won?Youl?Ryu,Moon?Yup?Jang,Moo?Hwan?Cho  author-information"  >  author-information__contact u-icon-before"  >  mailto:mhcho@yumail.ac.kr"   title="  mhcho@yumail.ac.kr"   itemprop="  email"   data-track="  click"   data-track-action="  Email author"   data-track-label="  "  >Email author
Affiliation:(1) School of Chemical Engineering & Technology, Yeungnam University, Gyongsan, 712-749 Gyongbuk, Korea
Abstract:A visual method for the selective screening of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase. Syringaldazine and Acid Red 264 were able for the detection of both the MnP and laccase, and the LiP and laccase, respectively, and a combination of these two additives was able to detect each of the ligninases produced by the WRF on solid media.
Keywords:lignin peroxidase  manganese peroxidase  laccase  color additives  white rot fungi
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