The selective visualization of lignin peroxidase,manganese peroxidase and laccase,produced by white rot fungi on solid media |
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Authors: | Won?Youl?Ryu Moon?Yup?Jang Email author" target="_blank">Moo?Hwan?ChoEmail author |
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Institution: | (1) School of Chemical Engineering & Technology, Yeungnam University, Gyongsan, 712-749 Gyongbuk, Korea |
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Abstract: | A visual method for the selective screening of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated
by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR
could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase. Syringaldazine and Acid
Red 264 were able for the detection of both the MnP and laccase, and the LiP and laccase, respectively, and a combination
of these two additives was able to detect each of the ligninases produced by the WRF on solid media. |
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Keywords: | lignin peroxidase manganese peroxidase laccase color additives white rot fungi |
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