Neuronal Localization of Ca2+-dependent Protein Phosphorylation in Brain |
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Authors: | Werner Sieghart Howard Schulman Paul Greengard |
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Institution: | Department of Pharmacology, Yale University School of Medicine, New Haven, Connecticut 06510, U.S.A. |
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Abstract: | Abstract: The cellular localization of two Ca2+-dependent protein phosphorylation systems was investigated using the kainic acid lesioning technique for the selective destruction of neurons. In one of these systems, a crude synaptosomal (P2) fraction was preincubated with 32Pj for 30 min; the phosphorylation of several proteins was increased during a short subsequent incubation with veratridine plus Ca2+. In the second system, crude synaptosomal membranes isolated from the P2 fraction were incubated with γ-32P]ATP; in this system, the phosphorylation of several proteins was increased in the presence of a "calcium-dependent regulator" plus Ca2+. Kainic acid lesioning greatly reduced the amount of Ca-+-dependent protein phosphorylation in both systems. The results indicate a predominantly neuronal localization for both Ca2+-dependent protein phosphorylation systems. |
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