Relationship of glutamate dehydrogenase levels to free amino acids, amides and ammonia in excised oat leaves

Levels of glutamate dehydrogenase (GDH) increase 12 fold indetached oat leaves during 96 hr incubation with 15 mM ammonia.The slight elevation of GDH detected within the first 24 hrwas followed by increasing rates of enzyme production in subsequentperiods. Rapid increases in free ammonia and amino acids witha marked synthesis of glutamine and decreased of glutamate andaspartate were observed during the initial stages of ammoniumassimilation. (Received December 26, 1973; )