Affinity of fibroblast growth factors for beta-cyclodextrin tetradecasulfate |
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| Authors: | S N Yuen J Folkman P B Weisz M M Joullie W R Ewing |
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| Institution: | Department of Surgery, Children's Hospital, Boston, Massachusetts. |
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| Abstract: | beta-Cyclodextrin tetradecasulfate was found to have a very strong affinity for fibroblast growth factor (FGF) and could substitute for heparin in FGF purification. Basic FGF was purified about 200,000-fold from a rat chondrosarcoma using a method of biaffinity chromatography in which the beta-cyclodextrin tetradecasulfate polymer was mixed with copper-Sepharose. This method takes advantage of the strong affinity of FGF for both beta-cyclodextrin tetradecasulfate and copper. |
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