Catabolite inactivation,cyclic AMP and protein phosphorylation in the methylotrophic yeastHansenula polymorpha |
| |
Authors: | Klaus H Hofmann Elke Polnisch |
| |
Institution: | (1) Institut für Angewandte und Technische Mikrobiologie, Ernst-Moritz-Arndt-Universität Greifswald, 0-2200 Greifswald, Germany |
| |
Abstract: | The inactivation of the peroxisomal enzyme alcohol oxidase and the cytoplasmic enzymes fructose-1,6-bisphosphatase, malate dehydrogenase and phosphoenolpyruvate carboxykinase was found to occur after addition of glucose to methanol-grown cells of the yeastHansenula polymorpha. The concentration of cyclic AMP increased nearly twofold within 3 min under the same conditions. In crude extracts ofH. polymorpha about 20 proteins are phosphorylated by cyclic AMP dependent protein kinases, among them also fructose-1,6-bisphosphatase. No phosphorylation of the alcohol oxidase protein could be detected. From this fact, it was concluded that the inactivation of the peroxisomal alcohol oxidase is independent of cyclic AMP-dependent protein phosphorylation. |
| |
Keywords: | alcohol oxidase catabolite inactivation cyclic AMP Hansenula polymorpha protein phosphorylation |
本文献已被 SpringerLink 等数据库收录! |
|