Protein-filled polyelectrolyte microcapsules in the design of enzymic microdiagnostics |
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Authors: | B I Sukhorukov S A Tikhonenko E A Saburova A V Dubrovskii Yu N Dybovskaya L I Shabarchina |
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Institution: | (1) Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia |
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Abstract: | Encapsulation of enzymes (lactate dehydrogenase and urease) in polyelectrolyte shells was assessed with a view to designing enzymic microdiagnostics for low-molecular compounds in native biological fluids. Polyelectrolyte microcapsules were prepared with two polyanions poly(styrenesulfonate) PSS and dextran sulfate DS] and two polycations poly(allylamine) PAA and poly(diallyldimethylammonium) PDADMA]; calcium carbonate microspherulites with embedded enzymes served as “cores.” It was demonstrated that the main problem in making such a biosensor is to select a pair of oppositely charged polyelectrolytes that would be optimal for enzyme functioning. The best pairs were PAA/DS and PAA/PSS for lactate dehydrogenase, and PSS/PAA and PSS/PDADMA for urease. We designed and prepared enzyme-containing microcapsules differing in polyelectrolyte composition and number of layers, and investigated their properties. |
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Keywords: | enzymes polyelectrolytes nanocapsules and microcapsules biosensors urease lactate dehydrogenase |
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