| 酵母双杂交筛选与甲状旁腺素1型受体相互作用的蛋白 |
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| 引用本文： | 刘俊丽,冯凯,路浩军,秦亚亚,张建中. 酵母双杂交筛选与甲状旁腺素1型受体相互作用的蛋白[J]. 生物技术通讯, 2010, 21(2): 188-191. DOI: 10.3969/j.issn.1009-0002.2010.02.010 |
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| 作者姓名： | 刘俊丽 冯凯 路浩军 秦亚亚 张建中 |
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| 作者单位： | 解放军306医院,病理实验科,北京,100101 |
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| 基金项目： | 306医院院级课题，总后司令部及卫生部课题 |
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| 摘 要： | 目的:采用Clontech公司第3套酵母双杂交系统,筛选与甲状旁腺素1型受体(PTH1R)相互作用的蛋白。方法:将全长PTH1R基因克隆到载体pGBKT7作为诱饵,用LiAc介导将诱饵蛋白质粒pGBKT7-PTH1R和人肾cDNA文库质粒转化到酵母菌AH109中,检测Mel1报告基因的表达,并进行相互作用的验证。结果:测序和相互作用验证结果表明SNAPIN在酵母系统中能特异地与PTH1R相互作用;根据对SNAPIN基因的功能分析,其可能与细胞内钙稳态的调控有关。结论:为进一步研究PTH1R促进骨肉瘤进展的机制提供了一定的线索。
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| 关 键 词： | 甲状旁腺素l型受体 酵母双杂交 相互作用 SNAPIN |
Screening of the Proteins Interacted with Parathyroid Hormone Type 1 Receptor by Yeast Two-Hybridization |
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| LIU Jun-Li,FENG Kai,LU Hao-Jun,QIN Ya-Ya,ZHANG Jian-Zhong. Screening of the Proteins Interacted with Parathyroid Hormone Type 1 Receptor by Yeast Two-Hybridization[J]. Letters in Biotechnology, 2010, 21(2): 188-191. DOI: 10.3969/j.issn.1009-0002.2010.02.010 |
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| Authors: | LIU Jun-Li FENG Kai LU Hao-Jun QIN Ya-Ya ZHANG Jian-Zhong |
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| Affiliation: | LIU Jun-Li,FENG Kai,LU Hao-Jun,QIN Ya-Ya,ZHANG Jian-Zhong Department of Pathology , Experimental Medicine,306th Hospital of PLA,Beijing 100101,China |
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| Abstract: | Objective:To screen the proteins which can interact with parathyroid hormone type 1 receptor(PTH1R) by yeast two-hybridization system.Methods:The full long PTH1R gene was subcloned into the vector of pGBKT7 to construct the bait plasmid pGBKT7-PTH1R.A cDNA library from human kidney recombinant plasmids was as the target genes.They were simultaneously transformed into yeast cell AH109 by LiAc-mediated transformation method.And then,the expression of reporter gene Mel1 was tested.Results:SNAPIN could interact... |
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| Keywords: | SNAPIN |
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