The use of liposomes in aspergillus niger van tieghem mannosylation:in vitro studies

The use of dolichyl phosphate-containing liposomes in glycoprotein biosynthesis under conditions that control the transfer of exogenous dolichyl phosphate from liposomes to microsomes is described. The exogenously added dolichyl phosphate is available for mannosylation, and direct evidence for the role of dolichyl phosphate as intermediate in protein mannosylation was shown by use of liposomes containing dolichyl ¹⁴C]mannosyl phosphate.