Development of a real-time multiplex PCR assay for the detection of multiple <Emphasis Type="Italic">Salmonella</Emphasis> serotypes in chicken samples |
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| Authors: | Edel O'Regan Evonne McCabe Catherine Burgess Sheila McGuinness Thomas Barry Geraldine Duffy Paul Whyte Séamus Fanning |
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| Institution: | (1) Centres for Food Safety and Food-borne Zoonomics, UCD Veterinary Sciences Centre, University College Dublin, Belfield, Dublin 4, Ireland;(2) Ashtown Food Research Centre, Teagasc, Ashtown, Dublin 15, Ireland;(3) Department of Microbiology, National University of Ireland Galway, Galway, Ireland |
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| Abstract: | Background A real-time multiplex PCR assay was developed for the detection of multiple Salmonella serotypes in chicken samples. Poultry-associated serotypes detected in the assay include Enteritidis, Gallinarum, Typhimurium,
Kentucky and Dublin. The traditional cultural method according to EN ISO 6579:2002 for the detection of Salmonella in food was performed in parallel. The real-time PCR based method comprised a pre-enrichment step in Buffered Peptone Water
(BPW) overnight, followed by a shortened selective enrichment in Rappaport Vasilliadis Soya Broth (RVS) for 6 hours and subsequent
DNA extraction. |
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