Effects of an endothelial cell-conditioned medium on the hematopoietic and endothelial differentiation of embryonic stem cells |
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Authors: | Xuan Sun Lamei Cheng Huaxin Duan |
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Institution: | a Institute of Reproductive and Stem Cell Engineering, Central South University, 86 Xiangya Road, Changsha 410078, China b National Engineering and Research Center of Human Stem Cell, Changsha 410078, China c The People's Hospital of Hunan Province, Changsha 410005, China |
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Abstract: | We have examined the effect of mouse bone marrow endothelial cell-conditioned medium (mEC-CM) on hematopoietic and endothelial differentiation of mouse embryonic stem cells (mESCs). mEC-CM can efficiently promote the differentiation of mESCs into Flk+ cells and hematopoietic colony-forming cells. mEC-CM proved to be as potent as a cytokine cocktail comprised of VEGF, bFGF, IGF and EGF. After inducing mESCs with mEC-CM, cobblestone-like cells were mechanically selected and identified which had the ability to incorporate DiI-Ac-LDL. DiI-Ac-LDL-positive cells were endothelial-like cells due to their expression of CD31 and Flk1, ability to bind to UEA1 and capacity to form capillary-like tube structures on matrigel. In conclusion, mEC-CM can efficiently promote the differentiation of mESCs into endothelial cells and hematopoietic colony-forming cells. The differentiated endothelial-like cells can be isolated by using DiI-Ac-LDL labeling and mechanical selection. |
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Keywords: | Conditioned medium DiI-Ac-LDL Embryonic stem cells Endothelial cells Hematopoietic cells |
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