Recombinational hotspot activity of Chi-like sequences |
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Authors: | K C Cheng G R Smith |
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Institution: | 1. Fred Hutchinson Cancer Research Center 1124 Columbia Street Seattle, Wash. 98104, U.S.A.;2. Department of Pathology University of Washington Seattle, Wash. 98195, U.S.A. |
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Abstract: | Chi sites, consisting of the nucleotide octamer 5' G-C-T-G-G-T-G-G 3', stimulate coliphage lambda recombination mediated by the Escherichia coli RecBC recombination pathway. In a sensitive genetic assay using phage lambda crosses, three of four Chi-like sequences tested, namely 5' A-C-T-G-G-T-G-G 3', 5' G-T-T-G-G-T-G-G 3' and 5' G-C-T-A-G-T-G-G 3', had about 6%, 11% and 38% of full Chi activity, respectively. We conclude that certain Chi-like sequences manifest a spectrum of recombinational hotspot activities and may account for RecBC-mediated generalized recombination of lambda lacking Chi sites. |
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