Structural and serological characterization of the O-chain polysaccharide of Aeromonas salmonicida strains A449, 80204 and 80204-1 |
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| Authors: | Wang Zhan Vinogradov Evgeny Larocque Suzon Harrison Blair A Li Jianjun Altman Eleonora |
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| Affiliation: | Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6. |
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| Abstract: | The O-chain polysaccharide (O-PS) of Aeromonas salmonicida was studied by a combination of compositional, methylation, CE-ESMS and one- and two-dimensional NMR analyses. It was found to be a branched polymer of trisaccharide-repeating units composed of L-rhamnose (Rha), D-glucose (Glc), 2-acetamido-2-deoxy-D-mannose (ManNAc) and O-acetyl group (OAc) and having the following structure: CE-ESMS analysis of A. salmonicida cells from strains A449, 80204 and 80204-1 grown under different conditions confirmed that the O-PS structure was conserved. ELISA-based serological study with native LPS-specific antisera performed on the native O-PS and its O-deacetylated and periodate-oxidized derivatives confirmed the importance of the O-PS backbone structure as an immunodominant determinant. |
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| Keywords: | CE-ESMS, capillary electrophoresis-electrospray mass spectrometry COSY, correlated spectroscopy 1D, 2D, one- and two- dimensional dHexOAc, O-acetylated deoxy hexose ELISA, enzyme-linked immunosorbent assay GLC, gas-liquid chromatography Glc, glucose HMBC, heteronuclear multiple bond correlation HSQC, heteronuclear single quantum correlation LPS, lipopolysaccharide ManNAc, 2-acetamido-2-deoxy-mannose MS, mass spectrometry NMR, nuclear magnetic resonance NOESY, nuclear Overhauser effect spectroscopy OAc, O-acetyl O-PS, O-chain polysaccharide Hex, hexose HexNAc, 2-acetamido-2-deoxy-hexose Rha, rhamnose TOCSY, total correlated spectroscopy TSA, Tryptic Soy Agar TSB, Tryptic Soy Broth |
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