1.Physical Faculty,Moscow State University,Moscow,Russia;2.Engelhardt Institute of Molecular Biology,Russian Academy of Sciences,Moscow,Russia;3.Fiber Optics Research Center,Russian Academy of Sciences,Moscow,Russia
Abstract:
The cleavage of dsDNA fragments in aqueous solution after irradiation with UV laser pulses at 193 nm has been studied. Samples
were investigated using polyacrylamide gel electrophoresis. The intensity of damage of particular phosphodiester bond after
hot alkali treatment was shown to depend on the base pair sequence. It was established that the probability of cleavage is
twice higher for sites of DNA containing two or more successively running guanine residues. A possible mechanism of damage
to the DNA molecule connected with the migration of holes along the helix is discussed.