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New insights on the origin of B chromosomes in <Emphasis Type="Italic">Astyanax scabripinnis</Emphasis> obtained by chromosome painting and FISH
Authors:Marcelo?Ricardo?Vicari  Helena?Flávia?de?Mello Pistune  Jonathan?Pena?Castro  Mara?Cristina?de?Almeida  Luiz?Antonio?Carlos?Bertollo  Orlando?Moreira-Filho  Juan?Pedro?M?Camacho  Email author" target="_blank">Roberto?Ferreira?ArtoniEmail author
Institution:Departamento de Biologia Estrutural, Molecular e Genética, Universidade Estadual de Ponta Grossa, Av. Carlos Cavalcanti, 4748, Ponta Grossa, PR 84030-900, Brazil.
Abstract:Chromosome painting (CP) with a probe of B chromosome obtained by microdissection and fluorescence in situ hybridization (FISH) with probes of As51 satellite DNA, C o t−1 DNA, and 18S and 5S rDNA confirmed sharing of some repetitive DNA but not rDNA between A and B chromosomes in the fish Astyanax scabripinnis. Meiotic analysis revealed a pachytene B chromosome bivalent nearly half the size of its mitotic configuration, suggesting a self-pairing of B chromosome arms. Such an isochromosome nature of somatic B chromosome was further evidenced by CP and FISH. All the findings obtained suggest (i) intraspecific origin of B chromosome, and (ii) evolutionary enrichment of repetitive DNA classes, especially those contained in the C o t−1 and the As51 probes, in B chromosome. However, the precise origin of B chromosome in the present species remains to be elucidated by further molecular cytogenetic analysis because of painting of some A chromosome regions with the B chromosome-derived probe.
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