Molecular cloning of, and phylogenetic analysis of, an actin in Naegleria fowleri |
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| Authors: | Suzanne Gorospe R. Neal Band Will J. Kopachik |
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| Affiliation: | Department of Microbiology, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695-7624, USA; Department of Food Science, Southeast Dairy Foods Research Center, North Carolina State University, Raleigh, NC 27695-7624, USA |
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| Abstract: | Abstract Two strains of Lactobacillus acidophilus Group A1, the neotype ATCC 4356 and a human isolate NCFM-N2, widely used as a dietary adjunct in milk and cultured dairy products, were transformed with plasmid DNA by electroporation. The transformation characteristics exhibited by the two L. acidophilus strains were found to differ markedly even though they appeared similar at the genomic level based on the DNA patterns of Sma I restriction fragments. To our knowledge, this is the first report of a consistent, reproducible transformation system of Lactobacillus acidophilus strains comprising the A1 DNA homology group. |
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| Keywords: | Transformation Electroporation Lactobacillus acidophilus Plasmid |
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